Linked or semi-linked downstream processing delivers significant advancements to productivity, Expense, timelines, and facility footprint. Our versatile portfolio allows you end up picking an intensification method that aligns with the special approach plans.
You'll find selected brands of hybrid or enforced silica centered particles of RP columns which can be used at Serious pH situations. The use of Excessive acidic situations can be not proposed, as Additionally they could possibly hydrolyzed and also corrode the inside partitions with the metallic areas of the HPLC products.
If stationary phase inside the column is really a liquid, the column is considered a bonded column. Bonded columns contain a liquid stationary phase bonded to some bought assist, and that is again typically silica or alumina. The worth from the frequent C described in the van Deemter equation is proportional, in HPLC, to the diameter with the particles that represent the column's packing material.
Reversed period columns are pretty challenging to damage in comparison to usual silica columns, due to the shielding effect of the bonded hydrophobic ligands; nonetheless, most reversed stage columns consist of alkyl derivatized silica particles, and they are vulnerable to hydrolysis from the silica at Extraordinary pH circumstances within the cellular phase. Most types of RP columns should not be utilised with aqueous bases as these will hydrolyze the underlying silica particle and dissolve it.
Large number of processes and circulation path configurations accessible to structure your own multi-step process.
A rise in counter ion (with respect on the purposeful teams in resins) focus cuts down the retention time, mainly because it results in a powerful Competitors While using the solute ions. A lessen in pH reduces the retention time in cation exchange although a rise in pH lowers the retention time in anion Trade.
Also, dimension exclusion HPLC is utilized from the characterization of nanoparticles and also the analysis of complex Organic samples.
Repairs are concluded employing only legitimate Agilent areas that in shape your instrument and maximize performance.
Lets simultaneous and constant Procedure of up to 3 chromatography separations. These may be Element of a batch and/or multi-column procedure
Be part of Sartorius as we examine ways to transfer a standalone batch mAb chromatography approach to some connected DSP.
Allows higher productivity than classic chromatography, minimized buffer and resin volumes and lower click here resin costs
Enables higher productiveness than regular chromatography, diminished buffer and resin volumes as well as reduce resin costs
Sartorius chromatography click here consumables address the full array of separation systems and methodologies obtainable to support any method and any mo...
In the HPLC column, the parts of the sample separate centered on their own differing interactions with the column packing. If a species interacts extra strongly Using the stationary stage in the column, it can invest far more time adsorbed for the column's adsorbent and can hence have a increased retention time. Columns could be full of solids including silica or alumina; these columns are referred to as homogeneous columns.